ABSTRACT

The traditional medicine involves the use of different plants and their bioactive constituents to treat disease and infection. Acacia nilotica is a multipurpose plant belonging to the family Fabaceae. It has been used traditionally to treat infections. The current study aimed at investigating presence of phytochemical constituents and antimicrobial activity of Acacia nilotica leaves extract (Methanol, Chloroform and Water) against multi drug resistant Escherichia coli, Pseudomonas, Enterobacter, Staphylococcus aureus and Candida albican. Extract of Acacia nilotica were prepared using Methanol, Chloroform and Water were screened for the antimicrobial activity using agar well diffusion method. The crude extract was further subjected to phytochemical analyses and Thin layer chromatography (TLC). Methanolic extract of leaves of Acacia nilotica were more active against all five pathogens. It gives highest 24mm zone of inhibition (ZOI) against Pseudomonas. Chloroform extract of leaves of Acacia nilotica only active against Staphylococcus aureus. It gives 13mm zone of inhibition (ZOI) against Staphylococcus aureus. Water extract of leaves of Acacia nilotica only active against Staphylococcus aureus. Water extract gives highest 16mm zone of inhibition (ZOI) against Staphylococcus aureus, whereas Methanolic and Chloroform extract gives zone of inhibition (ZOI) 15mm and 13mm. The Methanol, Chloroform and Water extract of leaves were used for the phytochemical analysis to find out the phytochemical constituents in the Acacia nilotica. The results of the phytochemical analysis of the Acacia nilotica showed that alkaloids, glycoside, seponins, phytosterol, phenol, tannin, flavonoid, protein and Quinone’s present in leaves extract. Keywords: Phytochemical analysis, Acacia nilotica, Medicinal plants, Antimicrobial activity, Escherichia coli, Pseudomonas, Enterobacter, Staphylococcus aureus and Candida albican
Publication date: 01/09/2020

https://ijbpas.com/pdf/2020/September/MS_IJBPAS_2020_5172.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.9.5172