ABSTRACT

Silver nanoparticles (AgNPs) have gained received great interest in cancer nanomedicine owing to its wide range of biomedical applications. The present study aimed to investigate the anticancer activity of AgNPs gel formulated with Aegle marmelos leaf extract against human melanoma cell lines SK-MEL-2. Biogenic synthesis of AgNPs was carried out using A. marmelos leaves extract acting as a reducing agent. Biosynthesized nanoparticles were characterized by a UV-spectroscopic technique, demonstrating a peak at 421 nm. Four batches of AgNP gel (A-D) were formulated using varying concentrations of carbopol 934 as a gelling agent and potential anticancer activity was evaluated against human melanoma cell lines SK-MEL-2 by Sulforhodamine B (SRB) assay. Among the four batches, AgNP gel A exhibited the highest percent control growth of 53.5% at 40 ?g/mL concentration, which is comparable to that shown by positive control ADR. AgNP gel A also exhibited significant anti-proliferative activity with LC50 value of 79.6 ?g/mL, which is greater than that of ADR (LC50 =50.5). Both the GI50 and TGI values for AgNP gel A were found to be <10 ?g/mL, which is similar to that of positive control ADR. Thus, it can be concluded that AgNP gel formulated from A. marmelos can be a potential anticancer agent for skin cancer. Keywords: Silver nanoparticles, Aegle marmelos, cytotoxicity, sulforhodamine B assay, anticancer, SK-MEL-2 cell lines
Publication date: 01/08/2020

https://ijbpas.com/pdf/2020/August/MS_IJBPAS_2020_5156.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.8.5156