EXTRACTION AND PURIFICATION OF PHYCOCYANIN FROM Spirulina platensis BY USING DIFFERENT METHODS: A REVIEW Authors: Panchal J and Shrivastav A*
ABSTRACT
Spirulina is a cyanobacteria known as blue green algae and consist of large amount of
protein. This algae has been used as source of protein and vitamins. Spirulina mainly known
for high content of Phycocyanin protein. Phycocyanin belong to the phycobiliprotein family,
which is characterized as blue colorpigment, light harvesting, soluble in water which has
high nutritional value, and antioxidant, anticancer and anti-inflammatory properties. It has
application in a food, cosmetic as well as pharmaceutical industry. This review article
describes the extraction of phycocyanin from spirulina platensis by using different
extraction methods, and purification of phycocyanin by different purification methods.
Extraction of phycocyanin carried out by different method includes physical, chemical, and
enzymatic method. Physical methods include homogenization and freeze thawing, chemical
method include inorganic acid and organic acid treatment and enzymatic method includes
lysozyme treatment. Purification of phycocyanin is carried out by different method steps
ammonium sulfate precipitation, dialysis, gel electrophoresis and ion exchange
chromatography. Present review will highlight the methods involved in extraction and
purification of phycocyanin.
Keywords: Phycocyanin, Spirulina, Extraction, Purification, Application Publication date: 01/07/2020 https://ijbpas.com/pdf/2020/July/MS_IJBPAS_2020_5117.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2020/9.7.5117