ABSTRACT

The objectives of this study were to establish the HPTLC phytochemical profiling and determine the antioxidant activity of ethanolic seed extract blend of Lepidium sativum and Trigonella foenum graecum. The extract was subjected to assess its antioxidant potential using in vitro system DPPH and NO radical scavenging assay. In the DPPH assay, extract blend has started showing free radical scavenging activity at a concentration of 50 ?g/mL (73.18±15%). The free-radical scavenging activity of extract blend was almost near to the standard ascorbic acid (88.2±02% at 50?g/mL concentration). Similarly, the extract blend also has exhibited significant free radical scavenging activity in NO assay from the concentration 100 ?g/mL (63.14±0.51%) compared to the standard ascorbic acid (92.78±0.02 % at 100 ?g/mL) which clearly indicating that the extract blend is also potent in scavenging free radicals in vitro. The ability of this extract blend to scavenge DPPH and NO could also reflect its ability to inhibit the stress and subsequently the nuero-degenarative diseases related to memory. It is concluded that the ethanolic seed extract blend of Lepidium sativum and Trigonella foenum graecum has strong antioxidant potential. These findings show that the polyphenolic constituents in the extracts are responsible for free radical scavenging capacity Keywords: Lepidium sativum, Trigonella foenum graecum, Antioxidant, DPPH, NO, HPTLC
Publication date: 01/07/2020

https://ijbpas.com/pdf/2020/July/MS_IJBPAS_2020_5102.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.7.5102