ABSTRACT

Waste substrates such as peels and seeds are accumulated with several bioactive compounds that impart various pharmacological properties. Longan is a sub tropical fruit that is either consumed as fresh, frozen, canned or dried. In spite of being rich in several bioactive and non-nutritive compounds, the peels and seeds of this fruit is usually discarded. The objective of the present study was to compare the antioxidant activity of peel and pulp extracts of Dimocarpus longan. Total phenol and flavonoid content was estimated using Folin – Ciocalteau and Aluminum Chloride method respectively. Antioxidant activity was determined using DPPH, FRAP and Phosphomolybdenum assay. All the assays were carried out in triplicates. Bioactive compounds were analyzed using Gas Chromatography-Mass Spectrometry. Results indicated that the peel extract exhibited greater potential to scavenge DPPH free radicals when compared to pulp extract thereby indicating the use of waste substrates as free radical inhibitors.IC50 value of peel and pulp extracts were found to be 57.07?g/mL and 256.21?g/mL. Results of reducing power assays showed that peel extract exhibited greater reducing capacity than the pulp extract. Gas Chromatography-Mass Spectrometry analysis showed the presence of 14 bioactive compounds in peel extract and 12 bioactive compounds in pulp extract. Findings of the study indicate that peel extract possessed relatively higher antioxidant activity thereby highlighting the possibility of utilizing fruit peels as a natural source of antioxidant. Keywords: fruit peel, waste substrates, polyphenols, antioxidant activity
Publication date: 01/06/2020

https://ijbpas.com/pdf/2020/June/MS_IJBPAS_2020_5071.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.6.5071