ANALYSIS OF OXALATE LEVELS IN BROCCOLI (Brassica oleracea, L.) AND CAULIFLOWER (Brassica oleracea. L vr botrytis sub var. Cauliflora DC) USING THE UV SPECTROPHOTOMETRY METHOD Authors: Emawati E , YULIANTINI A AND ELISEU
ABSTRACT
Vegetables are nutritious food containing vitamins and minerals needed by the body and
cheap prices. Brukoli and cabbage flowers are many vegetables containing vitamins A, B, C,
minerals, and calcium. In addition to vegetables that contain lots of nutrients, vegetables also
contain anti-nutrition, one of which is oxalic acid. Oxalic acid can interfere with the intestinal
lining if consumed and can be fatal if it is in high doses so it needs to be limited to the safe
range of 0.60-1.25 g per day. The purpose of this study is to determine the levels of oxalate in
broccoli and cabbage. flower. The method used is UV spectrophotometry with a wavelength
of 351 nm. This research begins with the validity of the method which includes linearity,
detection limits, quantization limits, precision and accuracy using addition methods. The
results of the validation of the calibration curve analysis method have a regression line
equation y = 0.0224 x + 0.2407 with a correlation coefficient of 0.993. The detection limit
and the quantization limit can be calculated statistically respectively 1.0045 and 3.4860.
Variance coefficient of 1.7570 and percent recovery, respectively 87, 89 and 92%. The
results of this study showed that the levels of oxalate in broccoli were 1.3797 mg / 100 g and
cauliflower was 0.1707 mg / 100 g. Oxalate levels in broccoli and cauliflower are at safe
limits.
Keywords: Broccoli, cauliflower, oxalate, UV spectrophotometry Publication date: 01/06/2020 https://ijbpas.com/pdf/2020/June/MS_IJBPAS_2020_5056.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2020/9.6.5056