Agarwood plant (Aquilaria malaccensis Lam) is one of the forest plants that has medicinal and cosmetic benefits. When skin is broken, it will be easily infected by bacteria, one of which is Staphylococcus aureus. This study aimed to evaluate the antibacterial activity of the extract and fraction of Agarwood leaves against Staphylococcus aureus. The extract and fraction of Agarwood leaves were assayed for antibacterial activity using microdilution and bioautography methods. The extraction was performed by using maceration method with ethanol 96% as the solvent. The fractionation was carried out using liquid-liquid extraction method with n-hexane, ethyl acetate, and methanol-water as the solvents. The results showed that MIC value obtained for the extract,n-hexane fraction, ethyl acetate fraction,and methanol-water fraction was 32 ?g/mL, 16 ?g/mL, 32 ?g/mL, and 32 ?g/mL, respectively. In addition, MBC value for the extracts, n-hexane fraction, and ethyl acetate fraction was 512 ?g/mL, 512 ?g/mL, and was 256 ?g/mL. The results indicate that the ethyl acetate fraction has stronger antibacterial activity than the extract, the n-hexane fraction and the methanol-water fraction. The bioautography test found the compounds that are assumed to be active as antibacterial, namely phenolic compounds.
Keywords: Agarwood Leaves, Antibacterial, Staphylococcus aureus, Microdilution Method, MIC Value, MBC Value, Bioautography Test
Publication date: 01/05/2020
https://ijbpas.com/pdf/2020/May/MS_IJBPAS_2020_5042.pdf
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https://doi.org/10.31032/IJBPAS/2020/9.5.5042
