Empirically, Kabau seeds (Archidendron bubalinum (Jack.) I.C.Nielsen) are used by
Sumatran people as antidiabetic drugs. This study aims to investigate the antidiabetic activity
of ethanol extract of Kabau seeds. The methods used in this study were insulin resistance and
insulin deficiency by using Swiss Webster mice, and ?-glucosidase enzyme inhibition. The
insulin resistance method was carried out preventively by inducing Lipofundin® 30 mL/Kg
body weight (bw) and fructose 0.52g /Kg bw for 28 days. The insulin deficiency mice was
induced by alloxan monohydrate at dose 55-60 mg / Kg bw and blood glucose levels were
measured on days 0, 3, 6, 9, 12, and 15. The animals were divided into 6 groups: negative
control, positive control, comparison group, Ethanol Extract of Kabau Seeds (EEKS) 95
mg/Kg bw, EEKS 190 mg/Kg bw, and EEKS 380 mg/Kg bw. Inhibition of the ?-glucosidase
enzyme was carried out at extract concentrations of 50, 100,150, 200, 250, 300, 350, 400,
450, and 500 ppm, and the absorbance was measured by using a micro-reader at a wavelength
of 425 nm. In the insulin resistant method, the values of constant insulin tolerance test (CITT)
for negative control, positive control, metformin 65 mg/Kg bw, EEKS 95 mg/Kg bw, EEKS
190 mg/Kg bw, and EEKS 380 mg/Kg bw obtained were 1.58, 0.45, 1.89, 1.75,1.82, and
1.62, respectively. In the insulin deficiency method, all doses could reduce blood glucose
levels on the 6th day. In conclusion, the results of this study show that ethanol extract of
Kabau seeds increases insulin sensitivity and insulin secretion at best dose of 190 mg/Kg bw.
Keywords: Kabau Seeds, Archidendron bubalinum (Jack.) I.C.Nielsen, diabetes mellitus
Publication date: 01/05/2020
https://ijbpas.com/pdf/2020/May/MS_IJBPAS_2020_5041.pdf
Download PDF
https://doi.org/10.31032/IJBPAS/2020/9.5.5041
