ABSTRACT

An isocratic reverse phase liquid chromatography (RP-HPLC) method has been developed and subsequently validated for the determination of Bicalutamide in pharmaceutical formulation. In this method Shimadzu technologies Phenomenex (250×4.6mm; particle size 5?m) column with mobile phase consisting of 0.5g of sodium dihydrogen phosphate buffer (pH adjusted to 2.9 with ortho phosphoric acid) and acetonitrile in ratio of 70:30 v/v was used. The detection wavelength is 271nm, flow rate 1.0 ml/min and the retention time was 9.302 min. The linearity was found in the range of 50?g/ml to 250?g/ml and shows a correlation coefficient of 0.998. Cefazolin sodium exhibits antibiotic activity. Analysis of cefazolin sodium was done by different analytical techniques like UV, HPLC. In UV the absorbance of the solution was observed over a spectral range from 200nm to 400nm using 0.1 M NaHCO3 solution as blank which shows maximum at 257nm. In this gradient method Shimadzu technologies Inertsil (150x4.6mm; particle size 5?m) column with mobile phase consisting of 6.8g potassium dihydrogen ortho phosphate buffer(PH adjusted to 6.8 0.05 with sodium hydroxide) and 3.4g of potassium dihydrogen ortho phosphate in water was taken and is mixed with acetonitrile in the ratio of 80:20v/v was used. The detection wavelength is 254nm, column oven temperature is 35°C, flow rate 1.5ml/min, retention time is 35.278 mins. Hence, an optimized, accurate, sensible, precise method was provided by which we can determine Bicalutamide in pure and pharmaceutical formulations in a cost efficient manner by RP-HPLC and analysis of cefazolin sodium was done by UV and HPLC. Key words: Bicalutamide, HPLC, Validation, Cefazolin sodium, UV
Publication date: 01/05/2020

https://ijbpas.com/pdf/2020/May/MS_IJBPAS_2020_5028.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.5.5028