ABSTRACT

The aim of this study includes isolation, characterization and molecular identification of Salmonella serovars of Punjab Pakistan. Bacterial strain was isolated and characterized with various biochemical tests and confirmed through molecular characterization. Using universal primers bacterial 16S rRNA gene was amplified. Compare the sequence in NCBI sequence database with amplified 16S rRNA gene sequence. The bacterial strain was amplified as Salmonella typhi. Phylogenetic and molecular evolutionary analysis was conducted by 16S rRNA sequencing. Salmonella typhi is a gram negative, rode shaped bacteria which forms black center colony on SS agar. The sequence when submitted to NCBI gene bank database using BLAST showed 99% maximum identity with all closely related taxa. Keywords: Salmonella, 16S rRNA, Isolation, characterization, NCBI.
Publication date: 01/05/2020

https://ijbpas.com/pdf/2020/May/MS_IJBPAS_2020_5005.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.5.5005