ABSTRACT

The ribonucleases perform various cellular functions. In addition, they exhibit certain therapeutic properties. Especially, the bacterial ribonuclease (binase) is a potent anti-cancer and antiviral agent. In the present paper, the sequence of binase enzyme was collected from UniProt webserver, based on which binase enzyme model (three-dimensional) was built in Phyre2 server. The model quality of binase enzyme was evaluated and validated using Verify3D and PROCHECK servers. Further the activity of binase can be enhanced using sophisticated computational tools. Such binase enzyme with improved activity can be produced in the laboratory by making corresponding changes in binase gene through site-directed mutagenesis in microbes. Key words: Ribonuclease, Binase, Anti-cancer, Binase enzyme model, Model quality
Publication date: 01/04/2020

https://ijbpas.com/pdf/2020/April/MS_IJBPAS_2020_50121.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.4.5012