ABSTRACT

The purpose of this work is to develop an accurate and precise HPLC method for the determination of Hydrochlorothiazide and Olmesartan Medoxomil in solid dosage form. Separation of the drug was achieved on an inertsil ODS 3V 150x4.5 column using a mobile phase consisting of 0.01M pH 3.0 phosphate buffer and acetonitrile by using a gradient programmer. The flow rate was 1.2ml/min and the detection wavelength was 262nm. The linearity was observed in the range of 15-90p-pm for Hydrochlorothiazide and 24-144ppm for Olmesartan Medoxomil with a correlation coefficient of 0.999 and 0.999 respectively. The impurities of Hydrochlorthiazide and Olmesartan Medoxomil were optimized and separated. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine control analysis of Hydrochlorothiazide and Olmesartan Medoxomil in solid dosage form by RP-HPLC. Keywords: Hydrochlorothiazide, Olmesartan Medoxomil, impurity profiling, dosage form RPHPLC, validation
Publication date: 01/04/2020

https://ijbpas.com/pdf/2020/April/MS_IJBPAS_2020_5017.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.4.5017