ABSTRACT

Background: Pseudomonas aeruginosa and Escherishia coli are virulent organisms, their multi drug resistant strains are become hallmark to treat. Various herbal remedies have shown antibacterial activity against both of these organisms out of them Fenugreek seed extract and Nigella sativa seed extracts have shown predominance. Objective: To evaluate the efficacy at different concentrations of Fenugreek seeds and Nigella sativa extract against Pseudomonas aeruginosa, E. coli and their MDR strains and to compare their efficacy with Amikacin and Ciprofloxacin. Methodology: It was an in-vitro cross sectional study, conducted in October – December 2019 after the ethical approval, in department of Microbiology, Baqai University Karachi. Different concentration of seed extracts was prepared and their antibacterial effects were observed on growth plate and efficacy was measured by zone of inhibition in millimeters. Results: N. sativa when compared against fenugreek shown high efficacy at 3mg/ml concentration against both organisms nevertheless ciprofloxacin preserved greater (56mm) zone of inhibition in P. aeruginosa.Conclusion: N. sativa Seed extract at 3mg.ml concentration have superior efficacy then fenugreek seed extract at either concentration when analyzed against P. aeruginosa and E coli but ciprofloxacin remained highly sensitive against P. aeruginosa. Keywords: Fenugreek, N. Sativa, Comparative efficacy, control drugs
Publication date: 01/04/2020

https://ijbpas.com/pdf/2020/April/MS_IJBPAS_2020_5003.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.4.5003