ABSTRACT

BACKGROUND: Vitamin D has major role in the calcium homeostasis. Recent advancements in the field of medicine and science enable the researchers to discover the whole new dimensions regarding the role of vitamin D. Deficiency of vitamin D is often associated with the non-skeletal diseases. One of these is type 2 diabetes mellitus (T2DM) and thus, with the discovery of vitamin D receptors on pancreatic beta cells have even increased their interest in the role of vitamin D as a causative factor of T2DM. Moreover, oxygen intermediates (ROI) is considered as one major factor for the onset and development of diabetes supported by recent evidences also compel the scientists to look into this domain too. AIMS AND OBJECTIVES: Aim and objective of the current study includes the role of vitamin D and oxidative stress in the development and progression of type II diabetes mellitus. METHODOLOGY: For the current study fifty patients (n=50) with T2DM and thirty (n=50) age and sex matched controls were substituted. All of the research work was carried out after the approval from the research ethical committee of the Institute of molecular biology and biotechnology (IMBB), The University of Lahore. An informed consent was obtained from the patients before including them into the study. Five ml of blood was taken from cubital vein, centrifuged and stored at -70?C for further analysis. The samples were processed and analyzed for the estimation of Vitamin-D, 8-OHdG and MDA with the help of their respective methods. RESULT: Current study shows the significantly elevated levels of oxidative stress markers i.e., MDA (0.07±0.006, p=0.01) and 8-OHdG (0.07±0.006, p=0.01) as compared to the control group (0.06±0.01 and 0.58±0.001 respectively). Furthermore, with the increases of ROS there is a noticeable decrease in the vitamin D levels (17.26±5.23, p=0.021) as compared to the control group (32.26±4.56) in patients having type 2 diabetes mellitus. CONCLUSION: From the results of this study it can be concluded that vitamin D deficiency or elevated levels of reactive oxygen species may have a contributing role in the development and progression of type 2 diabetes mellitus. Hence, therapies including the vitamin D supplementation may have significant role in the prevention and treatment of type 2 diabetes mellitus. Keywords: Vitamin D, Type 2 Diabetes Mellitus, MDA, 8-OHdG
Publication date: 01/04/2020

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https://doi.org/10.31032/IJBPAS/2020/9.4.4978