ABSTRACT

The discovery of bioactive compounds from natural sources entails an extremely important process in drug development. It is well known that an enormous molecular diversity and biological functionality are two important features that distinguish plant extracts as a drug source from synthetic chemicals. The six wild plant species collected from the desert of Hail city, (KSA), cleaned, air dried, grinded then extracted with ethanol 70% (crude extract). the crude extracts were tested for their antioxidant (using 2, 2 diphenyl- 1-picrylhydrazyl (DPPH), 2, 2'- azino-bis{ethylbenzthiazoline-6-sulfonic acid (ABTS.)} methods) and anticancer activity (using MCF-7 and Hep.G2). in addition to identify the active ingredients using GCMS. The antioxidant and anticancer activities of the crude extract exhibited the highest effect especially against ABTS radical (as antioxidant) and MCF-7 cell line (as anticancer). The extracts of Tamarix sp, Atriplex farinosa and Artemisia herba-Alba appeared highest activity with (99.56±2.81, 84.61±1.76 and 90.32±2.04 % at 200 ug/ml against ABTS), when compared with BHT and Vit. C as antioxidant standards (90.82±0.68 and 93.2±2.5 at 200 ug/ml against ABTS)) Moreover, these extracts gave (IC 50 with 44.2±1.7, 31.4±9.6 and 48.9±3.9 ug/ml respectively against MCF-7) when compared with DOX as anticancer standard. This activity was concentration dependent. The GC-MS results showed that there are 8 to 21 active compounds found in plant extract (6 samples) which revealed antioxidant and anticancer activities and the highest number of bioactive compounds found in extract no. 5 and 6. Also, the GC-MS exhibited that most of bioactive compounds undergo under three groups of secondary metabolites (Phenolics, Flavonoids and terpenoids) which responsible for the biological activities of plant species extracts under study. In conclusion, the study illustrated the potential of Tamarix sp, Atriplex farinosa as a valuable resource for natural compounds of desirable medicinal properties especially as antioxidants and anticancer. Keywords: Wild plants - Anticancer – Antioxidant -Hail region – GC-MS
Publication date: 01/03/2020

https://ijbpas.com/pdf/2020/March/MS_IJBPAS_2020_4996.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2020/9.3.4996