ABSTRACT

The needs of antioxidants are increasing to prevent the effects of free radicals. Natural antioxidants can be developed from medicinal plants, one of them is Pelawan Plant. This study aims to determine antioxidant activity, total flavonoids, total phenolics, and total carotenoids of Pelawan plant extracts. The extraction was carried out by reflux method using various solvent polarity such as n-hexane, ethyl acetate, and ethanol. Antioxidant activity was qualitatively analyzed using thin layer chromatography and visible UV-ray spectrophotometry with DPPH free radical scavenging and CUPRAC methods. Determination of total flavonoids, total phenolics and total carotenoids was carried out using UV-Visible spectrophotometry. The results of antioxidant activity test by using a DPPH showed that ethanol extract of leaves of Pelawan plant had the strongest activity with IC50 values of 17.68 ?g/mL. The results of antioxidant activity test by CUPRAC method showed that ethanol extract of leaves of Pelawan plant had the lowest capacity with EC50 value of 14.74 ?g / mL. The highest total flavonoid content was found in ethyl acetate extract of stems of Pelawan plant (4.8237 mg QE/100 mg), the highest total phenolic content was in ethanol extract of leaves of Pelawan plant (13.3625 mg GAE/100 mg) and the highest total carotenoid content was in n-hexane extract of leaves of (8.3297 mg BE/100 mg). In conclusion that the ethanol extract of leaves of Pelawan plant had very strong antioxidant activity. Keywords: Antioxidant, Carotenoids, CUPRAC, DPPH, Tristaniopsis obovata
Publication date: 01/03/2020

https://ijbpas.com/pdf/2020/March/MS_IJBPAS_2020_49271.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.3.4927