ABSTRACT

Ehretia microphylla, Dipteracanthus patulus and Hydnocarpus laurifolia is a main constituent of ethnomedicinal formulations used in Indian traditional medicine, there is very minimal systematic investigation done on this plant, it is not sufficient to support its traditional claim. The current study was attempted to assess the phytochemical and antioxidant activity of the Ehretia microphylla, Dipteracanthus patulus and Hydnocarpus laurifolia. The ABTS radical scavenging assay, DPPH radical scavenging assay, FRAP assay and Phosphomolybdate assay were performed for the confirmation of antioxidant activity. The Petroleum ether, Chloroform, Ehyl acetate and Methanolic extracts of these three plants were evaluated for the preliminary Phytochemical study which confirms the presence of various phytochemicals such as Alkaloids, Flavonoids, Tannins, Saponins, Glycosides, and Phytosterols. Petroleum ether extract of all the three plants do not have these secondary metabolites, so that excluding this petroleum ether extract for quantification of flavonoids and phenolic contents using spectrophotometer. The ethyl acetate and chloroform extracts of all the three plants having higher flavonoid content since further antioxidant activity was evaluated in both ethyl acetate and chloroform extracts of three plants. All the plant extracts shows good antioxidant activity and from the results of the current study the ethyl acetate and chloroform extracts of Ehretia microphylla, Dipteracanthus patulus and Hydnocarpus laurifolia were shows potent antioxidant activity which is similar to the standard. This study concluded that the flavonoids present in Ehretia microphylla, Dipteracanthus patulus and Hydnocarpus laurifolia may be the reason for antioxidant activity. Keywords: Flavonoid, DPPH, ABTS, Quercetin, Gallic acid
Publication date: 01/02/2020

https://ijbpas.com/pdf/2020/February/MS_IJBPAS_2020_4957.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.2.4957