ABSTRACT

Reason behind the study: The textile industry is indispensable for human civilization and hence it attracts several ideas for the improvement of its quality and durability. In this line, desizing by amylase has aroused a great deal of concern in the current textile industry. The objective of this study was production and optimization of amylase by Bacillus subtilis in order to exploit the potential of amylase in textile industries. Main findings: This work involves optimization of production of amylase using Bacillus subtilis and testing of their utility for cotton desizing. An Incubation period of 72 h, initial diluent pH of 7.0, 30°C incubation temperature, inoculum size of 1% (v/v) were optimum for the maximum amylase production yielding enzyme titer of 249 IU/ml. Supplementation of different carbon (1%), nitrogen (0.5%) and metal ions (1Mm) and detergents (1%) to the fermentation media suppressed amylase activity. Moreover, the desizing potential of ?-amylase was assessed via different standard assays. The crude enzyme showed efficient desizing of cotton fabric at pH 6.0, 65oC within 40 minutes with Tegewa rating 9, properties potential for desizing in textile industries. Conclusion: The purpose of this study is to produce amylase enzyme by Bacillus subtilis in submerged fermentation and optimizing its culture conditions to attain higher yield of amylase enzyme. The amylase obtained was further used to test its potential in desizing the cotton fabrics as that of the commercial enzyme. Keywords: Amylase, Bacillus subtilis, Optimization, Desizing, Cotton fabrics
Publication date: 01/02/2020

https://ijbpas.com/pdf/2020/February/MS_IJBPAS_2020_4956.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.2.4956