ABSTRACT

A comparative in vitro study was carried out for 3 tomato cultivars, Roma, Nagina and Cherry NARC. Two sources of explant were used; one was hypocotyl and another one was cotyledon leaves. MS media which was fortified with different concentration of phyto hormones was used to evaluate efficacy and the regeneration potential of the explant respectively. Among those three tomato varieties; tested for their callusing and regeneration potential, Roma responded as compared to Nagina and Cherry-NARC. Among all five different Medias tested in this regard MS media fortified with phyto hormones 2.5 mg/L BAP induced 76.6% callus from cotyledonary explants of Roma. MS media supplemented with IAA (0.2 mg/L) + BAP (2.0 mg/L) was found to be most efficient for shoot induction with an overall average of 27.78%. All the shoots were cultured on rooting media supplemented with MS media containing 0.2 mg/L of IAA. Keywords: Indole-3-Acetic Acid, 6-Benzylaminopurine, Cotyledon, Hypocotyl, MS media
Publication date: 01/02/2020

https://ijbpas.com/pdf/2020/February/MS_IJBPAS_2020_4936.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.2.4936