ABSTRACT

Mesenchymal stem cells (MSCs) are multipotent cells with an inborne ability of renewing themselves and differentiate into various cell types. Over the past five decades, MSCs have been extensively studied and have remained to be the focus of intense research. Despite promising potential, the therapeutic use of bone marrow (BM) MSCs has been limited due to difference in number of cells required for a therapeutic infusion (1–100 × 106 cells/kg of patient) and the number of cells that can be obtained from bone marrow samples. Several efforts have been carried out for the improvement of expansion techniques to increase the proliferative capability of MSCs. Zinc is one of those substances which is known for its cytoprotective and antioxidant properties. A scanty amount of studies have focused on the role of zinc in stem cells, none of them was on bone marrow derived stem cells. We designed an experiment to analyze the cytotoxicity of ZnCl2 on BM MSCs at late passages. We used various concentrations (10?M, 30?M, 50?M, 100?M & 300?M) of ZnCl2 for 48 hours and performed MTT assay. Cells treated with lower concentrations (10?M, 30?M, & 50?M) showed no cytotoxic effect; instead cell viability was found to be increased. With higher concentrations (100?M & 300?M) of ZnCl2, we observed significant cytotoxicity and a drastic decrease in cell viability. We advocate further studies to investigate the effects of zinc on cellular morphology, proliferation, and migration potential to counteract the limitation of BM-MSCs in a clinical setting. Keywords: Bone Marrow, Mesenchymal Stem Cells, Zinc Chloride, Cytotoxicity, Late Passages
Publication date: 01/01/2020

https://ijbpas.com/pdf/2020/January/MS_IJBPAS_2019_4918.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.1.4918