ABSTRACT

Rhizosphere soil of Pigeon pea crops of Gujarat region have been used to isolate actinomycetes as an elite source for the production of bioactive metabolites. The actinomycete strain S-9 was isolated during our systemic screening of rhizospheric actinomycetes and was identified as Streptomyces sp. Based on morphological, physiological and molecular approaches. Strain S-9 showed antifungal activity against Fusarium udum phytopathogenic fungi. Glucose, yeast extract, malt extract and starchas culture medium was selected for the production of Streptomyces sp. Plackett – Burman design (PBD) has been used to select yeast extract, malt extract and calcium carbonate (CaCO3) as parameters with significant effects on antifungal activity and a Box – Behnken design has been applied to further optimize the production medium. The analysis showed the optimal concentrations for the anti–F. udum activities of the tested variables were yeast extract 6.03 g/L, malt extract 7.05 g/L and CaCO3 4.59 g/L. Keywords: Streptomyces sp, Plackett – Burman design (PBD), Box – Behnken design Fusarium udum, bioactive metabolites
Publication date: 01/01/2020

https://ijbpas.com/pdf/2020/January/MS_IJBPAS_2019_4908.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.1.4908