ABSTRACT

H. pluvialis is a microalgae that can accumulate astaxanthin up to 5% dry weight of cells under stress conditions. The aim of study was to increase production of astaxanthin through physical, chemical induction of stress and the combination of physical and chemical induction of stress. H. pluvialis was cultivated using Walne's medium. On day 7, H. pluvialis was added with iron (II) heptahydrate with serial concentrations 450, 550, 650 ?M as chemical stress induction. Physical stress induction is carried out by irradiating UV-B with exposure time 30, 60, and 90 minutes. Physical and chemical stress induction by irradiating UV-B with 90 minutes exposure time and the addition of Fe2 550 ?M. Culture H. pluvialis was cultivated for 16 days to induce astaxanthin accumulation. H. pluvialis was extracted by maceration method using DMSO solvent. The results of quantitative analysis using a spectrophotometer, showed that UV-B irradiation for 90 minutes, the addition of Fe2 with a concentration of 550 ?M, and the combination of both can increase astaxanthin content 0.745 m /L, 0.926 mg/L and 0.771 mg/L respectively, than the control 0.342 mg/L. The results of the qualitative analysis using TLC silica gel F254 showed that the extract assumed contain astaxanthin. Keywords: Astaxanthin, H. pluvialis, UV-B, Fe2

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https://doi.org/10.31032/IJBPAS/2019/8.12.4883