ABSTRACT

Fowl cholera (FC) a contagious infection, remains a major threat to the poultry industry, particularly in developing countries including Pakistan. This study was carried out on suspected field cases to isolate and identify local strains of P. multocida. A sum of 25 samples was collected from suspected poultry flocks from different regions of Pakistan i.e. Lahore, Faisalabad, Mansehra, Abbottabad, Karachi, Lahore, and Vehari. The samples were processed and P. multocida strains were isolated. The primary identification of collected isolates was checked by colony morphology, microscopy, and biochemical characterization. The strains were molecularly characterized by PCR using 16S rRNA and P. multocida specific primers targeting OmpH gene. Samples positive for P. multocida showed gramnegative, cocco-bacilli, bipolar and non-motile characteristics. They were able to reduce nitrates but failed to produce hydrogen sulfide, consumed ornithine as a carbon source. The isolates consistently produced an acid while fermenting glucose and sucrose whereas lactose and maltose were not fermented. PCR successfully amplified the product of 500 bp and 993 bp against 16S rRNA and OmpH gene primers respectively. Results of molecular characterization confirmed the strains as P. multocida which can be used in the preparation of an inactivated vaccine against local isolates of fowl cholera. Keywords: Fowl cholera, OmpH gene, Poultry flocks, Pasteurella multocida

https://ijbpas.com/pdf/2019/December/MS_IJBPAS_2019_4874.pdf

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https://doi.org/10.31032/IJBPAS/2019/8.12.4874