ABSTRACT

Definite inhibition of proteases with their respective inhibitor is a special technique adopted for drug designing. PIs may also play their role as therapeutic agent for the treatment of cancer, cardiovascular and immunological diseases. Twenty-five bacterial strains were selected on the basis of their Protease Inhibitory activity, among them Bcaillus subtilis (M24) had highest protease inhibitory activity against trypsin. Then Protease Inhibitory activity was calculated before and after immobilization and there was a remarkable increase in Protease Inhibitory Activity against trypsin. Different Protease Inhibitory activities after immobilization against trypsin were 152 U/ml for Lewatite, 167U/ml for Dowex 80, 160U/ml for Dowex 66, 189 U/ml for Duolite, 175 U/ml for Amberlite, 207 Ulml for IR100, 157 U/ml for IR401. There were significant increase of protease inhibitory activity after immobilization, which were 9.4 fold increase for Lewatite, 10.8 fold for Dowex 80, 10 fold for Dowex 66, 12 fold for Duolite, 11 fold for Amberlite, 13 fold for IR100, 9.7 fold for IR401. Our final product, which is in the form of immobilized Protease Inhibitor of Bacillus subtilis are able to kill tumor protease and cancerous protein of human colon. Human colon has a natural habitat of Bacillus subtilis. The pro-biotic has ability to kill cancer protein and tumor protease due to the production of intracellular Protease Inhibitor in human colon. Bacillus subtilis produces alkaline Protease Inhibitor has strong tendency to fight against human cancer. Key words: Bacillus subtilis, Immobilization, Pro-biotic, Ion-exchange resins, Protease Inhibitor

https://ijbpas.com/pdf/2019/November/MS_IJBPAS_2019_4863.pdf

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https://doi.org/10.31032/IJBPAS/2019/8.11.4863