ABSTRACT

Different properties like anti-hypertensive, anti-microbial, anti-sickling, and anti-schistosomal (molluscicidal) have been found in medical plants. Medical plants have bioactive compounds which treat various ailments caused by microorganisms. Culture media was prepared in a conical flask, where 0.84 g of Muller Hinton Broth and 0.4 g of agar technical was added and the volume was made up to 1000 ml. Two bacterial strains of Escherichia coli and Pseudomonas aeruginosa were used. Disc diffusion method was designed to check over the activeness of Nannorrhops ritchiana. The minimum inhibitory concentration of the ethanolic extract of Nannorrhops ritchiana was found to be 100 mg, 200 mg, 300 mg, 400mg, 600 mg against Escherichia coli and 600 mg against Pseudomonas aeruginosa at different concentrations of leaf extracts ranging from 100 to 600 mg, Escherichia coli showed no Zone of inhibition except at 500 mg. On the other hand, Pseudomonas aeruginosa showed Zone of inhibition in concentrations ranging from 100 to 500 mg except at 600 mg no Zone of inhibition was seen. In conclusion, the plant showed minor activity against Escherichia coli but showed potent activityagainst Pseudomonas aeruginosa; and this study concluded that Nannorrhops ritchiana can be used as an antibacterial agent. Keywords: Escherichia coli, Pseudomonas aeruginosa, antibacterial activity, Nannorrhops ritchiana, Muller Hinton Broth

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https://doi.org/10.31032/IJBPAS/2019/8.11.4862