ABSTRACT

Uromastyx is genus of agamid lizard and known as spiny tailed lizard. By nature, Uromastyx are herbivorous but in younger age love to eat insects and small animals. Uromastyx borrows in desert, rocky and hilly areas. Size of Uromastyx is up to 91cm in adults and 10cm in neonates. Oil of Uromastyx is used for treatment of different disease. Aim of current study was to investigate fatty acid composition and seasonal variations in biochemical and hormonal profile. In two different seasons five samples of Uromastyx were collected and then fat extraction process done in laboratory, from fats of Uromastyx pure fats oil was extracted with Folch method by using chloroform and methanol in 2/1 ratio. GC-MS technique was used for analysis. From five samples, twenty-three fatty acids were identified and percentage of saturated fatty acids was 47.8% while unsaturated fatty acids were 30.4%. Benzenedicarboxlic, an aromatic fatty acid was present in all five samples of two seasons. Metabolites of vitamin B-12 were also identified in it. Hormonal studies of all three parameters luteinizing hormone, follicle stimulating hormone and cortisol were same except testosterone which was high in samples of December month. Study of fatty acids and hormonal profile of different seasons conclude that saturated fatty acids were higher as compared to unsaturated fatty acids. Sample of month December values of saturated fatty acids were lower as compared to October and similarly testosterone level were higher in December with all other parameters remained same. Keywords: Gas chromatography Mass spectrometry (GC-MS), Uromastyx, Vitamin B-12, Luteinizing Hormone (LH)

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https://doi.org/10.31032/IJBPAS/2019/8.11.4860