ABSTRACT

Normal pregnancy is associated with oxidative stress injury due to high energy demand of foetus during whole pregnancy and labour. Excessive oxygen demand leads to free radical generation and poor perinatal outcomes in most of the cases. Oxidative stress is responsible for a variety of degenerative processes in new-born babies in future. Measurement of oxidatively modified DNA, Lipids peroxidation products in biological samples may be helpful or will be served as predispositional important variables for future perinatal outcomes. Current study was designed to assess the differential expressions of malondialdehyde, isoprostanes and 8-Hydroxyguanosine in cord blood, serum and saliva immediately after labour in normal pregnancy. Thirty normal pregnant females and thirty clinically apparently healthy non-pregnant females aging from 25-35 years were recruited. Cord blood and saliva samples were collected and MDA, Isoprostanes and 8OHdG levels evaluated. Data was analyzed by performing Independent t-test through SPSS version 16.It was found that the malondialdehyde (MDA) levels raised significantly (p=0.038) in cord blood serum (1.43±0.0048 Vs 1.14±0.0027nmoles/ml), saliva (0.48±0.0020 Vs 0.031±0.0019nmoles/ml) and cord blood erythrocytes (0.34±0.0011 Vs 0.02±0.007nmoles/ml)from the normal pregnant females as compared to healthy non-pregnant females. Likewise the levels of isoprostanes and 8-hydroxy guanosine differed significantly (p=0.021) and (p=0.006) between subject and controls respectively. Current study suggest that due to increased lipidperoxidation the levels of their biomarkers also increased. Sothese markers have significant diagnostic value and predispositional variables for early detection of oxidative stress generated during pregnancy and used for future perinatal outcomes to assess the suspected complications. Keywords: MDA (Malondialdehyde), isoprostanes (IsP)), 8-OHdG (8-hydroxygunosine), Perinatal

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https://doi.org/10.31032/IJBPAS/2019/8.11.4859