EFFECT OF GRADED LEVELS OF DIETERY PLANT PROTEIN ON ELEMENTAL CONCENTRATION OF HYBRID (Catla catla? x Labeo rohita?) FROM PAKISTAN Authors: IQBAL R , NAEEM M* AND MASUD S
ABSTRACT
The written report under discussion was conducted in 90 days in order to analyse the impact of
three dietary protein feeds (15% CP, 20% CP, and 25% CP) on elemental concentration (Fe, K,
Na, Zn, Ca, Mg, Pb, Cd) in hybrid fry (Catla catla? x Labeo rohita?) by using Flame Atomic
Absorption Spectrometry. Criteria for preparation of feed was based on cost effectiveness and
local availability of ingredients. An increasing trend of essential elements was noted with
increasing protein level. Accumulation trend in all treatment groups in decreasing order is “K”>
“Ca”> “Na”> “Mg”> “Fe”> “Zn”> “Pb”> “Cd”. Regression analysis between different elements
and body weight exhibited significant correlation in group fed on 15% protein (T1) and 25%
protein (T3) except for Pb (Lead) and Cd (Cadmium) respectively, while the subjects fed on diet
containing 20% protein (T2) showed no correlations in different metals except in Ca (Calcium).
The study revealed that metals in different groups exhibited diverse allometric relation with
increasing body weight. Analysis of hybrid body length with total metal concentration revealed
that all metals evaluated in the study showed allometrically negative and non significant
correlation in all the groups fed on different diets. The multiple regression analysis showed no
relation among hybrid body weight and length against elemental concentration in all three
feeding groups except in some elements (Na, Pb, Cd). The results indicate, hybrid fed with plant
protein feeds showed the levels of elements (essential and non essential) within the permitted
levels of international standards.
Keywords: Crude protein (CP), hybrid, elemental concentration, Allometry, Multiple regression