IMPACT OF GRADED DIETARY PROTEIN ON HAEMATOOGICAL PARAMETERS OF GENETICALLY IMPROVED FARMED TILAPIA (GIFT)
Authors: KOUSAR A , NAEEM M* AND MASUD S

ABSTRACT
The objective of the present study was to evaluate the impact of graded levels of plant protein diets (15%, 20% and 25%) on haematological profile of mono sex (male) Genetically Improved Farmed Tilapia (GIFT), a developed strain of Nile tilapia (Oreochromis niloticus). Three treatment groups (T1=15%, T2=20% and T3=25%) were formed and experiment was carried out in duplicate. Five days old GIFT tilapia fingerlings of mean weight 0.24±0.03 (g) and length 2.01±0.27 (cm) were collected, acclimatized for two weeks, and transferred to hapas (8x6x3 ft) at random. Fingerlings were fed @ 10 % during acclimatization period and 5% up to the end of experiment. At the end of 90 days feeding trial, ten fishes were randomly selected from each treatment hapa for the estimation of haematology. Haematological parameters viz. WBCs, LYM, MON, GRA, RBCs, HGB, HCT, MCV, MCH, MCHC, PLT, MPV were studied. The RBCs values in T1, T2 and T3 were 0.93±0.30, 1.40±0.48 and 1.47±0.16 (106/?L) respectively. The WBCs values in T1, T2 and T3 were calculated as 55.51±10.25, 59.29±12.86 and 104.32±18.82 (103/?l) respectively. In T1, T2 and T3 the platelets count was estimated as 196.04±80.31, 225.87±59.32 and 290.00±186.00 respectively. Statistical relationship was studied applying linear regression analysis.RBCs, WBCs exhibited non-significant correlation against total length and weight in all groups.While, platelet count yielded non-significant correlation in T1, T2 and T3 with weight and length except for T2 which was found least significant against length. T3 fish fed at 25% CP exhibited more WBCs, RBCs and their indices with in the range as compared to T1 and T2 fish. Thus, protein diet (25%) is cost effective as well as keep the fish in good health condition. Keywords: Haematological profile, GIFT, Fingerling, Hapas, Graded levels of protein, Fish size

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https://doi.org/10.31032/IJBPAS/2019/8.11.4856