ABSTRACT

Infectious diseases have taken the lives of many people across the globe. Antibiotics can tackle with such disease causing bacteria but unfortunately antibiotic resistance is being increasing to such an extent that cases of multi drug strains of bacteria have been reported. To overcome such issues plant derived antibacterial are under research, therefore the aim of the present study was not only to evaluate the antibacterial activity of Pyrus pashia but also to potentiate the antibacterial effect of the plant extract using RSM. Therefore the optimized antibacterial activity of extract was tested against Escherichia coli and Bacillus subtilis through the measurement of zone of inhibition. All the bacteria were inhibited by the ethanolic plant extract. Quantitative phytochemical analysis was also done using in vitro assays. The phenolic content of plant extract was 14.26 mg of GAE/g; tannins came out to be 41.26 mg of GAE/g, alkaloids 45.26 mg of GAE/g, flavonoids 86.26 mg of GAE/g and carotenoids mg of GAE/g. The plant extract was known to possess antibacterial activity may be due to the presence of these phytochemicals as their antibacterial mode of action have been researched previously by the scientists. An overview of mode of action of these phytochemicals is also provided. This study concludes that Pyrus pashia possess antibacterial activity which can also be enhanced using specific values for temperature, pH and concentration. Keyword: Antibacterial activity, Pyrus pashia, Escherichia coli, Staphylococcus aureus

https://ijbpas.com/pdf/2019/October/MS_IJBPAS_2019_4821.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2019/8.10.4821