CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL OF Achillea biebersteinii
Authors: JEHAD AL-SHUNEIGAT , SAMEEH AL- SARAYREH1, MAHMOUD ALQUDAH2, YOUSEF AL–SARAIREH3, IBRAHIM AL-TARAWNEH4

ABSTRACT
This study was designed to examine the composition of Achillea biebersteinii essential oil and test its antioxidant activity. Gas chromatography–mass spectometry (GC-MS) was used to study essential oil composition. Antioxidant effectiveness of the essential oil was examined by three different radical scavenging methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, ferrous radical scavenging activity and 2,2'- azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) ferrous radical scavenging activity. Forty six components accounting for 100% of the oil were identified. The major identified compounds were trans-Sabinene hydrate acetate 30.09 %, Iso-Ascaridole 16%, ?-Terpinene 14.31%, p-Cymene 7.1%, cis-Carvone oxide 6.08 %, Terpinen-4-ol 2.75%, cis-Pulegol 2.58%, cis-Rose oxide 2.31%,1-Terpineol 1.93%, Z-?-Ocimene 1.9 %, trans- Verbenol 1.88%, trans-Piperitol 1.52%. Oxygenated monoterpene were the major oil components (70.22%), monoterpene hydrocarbon 26.95%, and sesquiterpene hydrocarbon 1.04%. The results of antioxidant activity tests show that Achillea biebersteinii essential oil exhibited less activity than control, ?-tocopherol and ascorbic acid, however it show a good antioxidant activity. Keywords: Achillea biebersteinii, essential oils, antioxidant, GCMS, DPPH, ABTS

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https://doi.org/10.31032/IJBPAS/2019/8.10.4839