CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL OF Achillea biebersteinii Authors: JEHAD AL-SHUNEIGAT , SAMEEH AL- SARAYREH1, MAHMOUD ALQUDAH2, YOUSEF AL–SARAIREH3, IBRAHIM AL-TARAWNEH4
ABSTRACT
This study was designed to examine the composition of Achillea biebersteinii essential
oil and test its antioxidant activity. Gas chromatography–mass spectometry (GC-MS) was
used to study essential oil composition. Antioxidant effectiveness of the essential oil was
examined by three different radical scavenging methods: 2,2-diphenyl-1-picrylhydrazyl
(DPPH) free radical scavenging activity, ferrous radical scavenging activity and 2,2'-
azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) ferrous radical scavenging
activity.
Forty six components accounting for 100% of the oil were identified. The major
identified compounds were trans-Sabinene hydrate acetate 30.09 %, Iso-Ascaridole 16%, ?-Terpinene 14.31%, p-Cymene 7.1%, cis-Carvone oxide 6.08 %, Terpinen-4-ol 2.75%,
cis-Pulegol 2.58%, cis-Rose oxide 2.31%,1-Terpineol 1.93%, Z-?-Ocimene 1.9 %, trans-
Verbenol 1.88%, trans-Piperitol 1.52%.
Oxygenated monoterpene were the major oil components (70.22%), monoterpene
hydrocarbon 26.95%, and sesquiterpene hydrocarbon 1.04%.
The results of antioxidant activity tests show that Achillea biebersteinii essential oil
exhibited less activity than control, ?-tocopherol and ascorbic acid, however it show a
good antioxidant activity.
Keywords: Achillea biebersteinii, essential oils, antioxidant, GCMS, DPPH, ABTS