ABSTRACT

Microalgae including Chlorella spp. have been applied in various biotechnological areas such as biofuels production, food industry, wastewater treatment etc. In this study, Random amplification of polymorphic DNA analysis using the polymerase chain reaction(RAPD-PCR) involving 4decamer random primers was used to evaluate the genetic variation in Chlorella spp. These twenty-eight (28) Chlorella spp. tested, were collected from freshwater environment in Abakaliki, Nigeria and cultivated in Bold basal medium (BBM). RAPD markers were used for amplification of the microalgal DNA isolated through cetyl trimethyl ammonium bromide (CTAB) method. The results showed that OPB-01, OPB-11 and OPH-08 primers exhibited maximum intra-species variation (100% polymorphism) while OPH-07showed the least (13%) polymorphism at amplicon levels. Despite morphological resemblance, a great deal of genetic polymorphism was observed among the Chlorella spp. Overall, the results revealed that the 4 RAPD bands are useful for genetic diversity studies of microalgae as there was species discrimination. Keywords: Microalgae, Polymorphism, Variation, RAPD markers, Chlorella spp.

https://ijbpas.com/pdf/2019/August/MS_IJBPAS_2019_4787.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2019/8.8.4787