PHENOTYPIC AND GENOTYPIC DIVERSITY ASSESSMENT OF JATROPHA CURCAS BY USING RAPD MARKERS TO IMPROVE BIODIESEL PRODUCTION Authors: Bharti A and Veeramani A*
ABSTRACT
Jatropha curcas L. is a multipurpose shrub has significant economic importance for its seed
oil, which can be converted into Biodiesel. In the present study, the genetic relationships of
three Jatropha curcas seeds collected from Labland Biotech Private Limited, Mysore (LB),
Gandhi Krishi Vigyan Kendra, Bangalore (GKVK) as well as from rural area of Jharkhand
(JH), were assessed by Random amplified polymorphic DNA(RAPD) analysis. Amplification
of genomic DNA using ten RAPD primers yielded 81 bands, of which 73 bands were
polymorphic. The amplification products ranged from 7-11 bands for different primers.
Among the chosen ten primers four of them, OPC-2, OPC-9, OPH-1 and OPQ-15, were
found to be polymorphic. RAPD analysis confirmed the genetic variability between the
accessions in relation to the variation in morphology and seed oil content, LB variety could
be considered superior as compare to other two accessions.
Keywords: Jatropha curcas, Genetic diversity, Biodiesel, RAPD, Fruit yield