ABSTRACT

Interleukin-1 alpha, TNF-? and IFN-? are the most important inflammatory cytokines that contribute to alopecia areata. The aim of this study was to assess the effect of naringenin in inhibition of inflammatory cytokines and controlling oxidative stress in patients with alopecia areata. Different concentrations of naringenin were added into keratinocyte cell culture medium, and the viability of keratinocyte cells was evaluated by MTT assay. RNA was extracted from treated keratinocyte cells and control, and cDNA was made. Next, RT-PCR and real time PCR were used for the detection of target genes and measuring gene expression levels, respectively. Moreover, DPPH method and H2O2-induced oxidative cell damage were used for assessing free radical scavenging activity of naringenin. The result showed that there was a significant difference in the level of gene expression of Interleukin-1 alpha, TNF-? and IFN-? in treated samples and control (p ? 0.05). In vitro free radical scavenging activity of naringenin was also confirmed. The result of in vivo test indicates that a gel containing naringenin is effective for the treatment of alopecia areata. This study provides scientific insights about the potential antiinflammatory and anti-oxidant activities of naringenin which can be used as effective flavanone for the treatment of inflammation indifferent types of autoimmune diseases. Keywords: Naringenin, Cytokines, Alopecia Areata

https://ijbpas.com/pdf/2019/August/MS_IJBPAS_2019_4624.pdf

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https://doi.org/10.31032/IJBPAS/2019/8.8.4624