ABSTRACT

Diabetes is a metabolic disorder characterized by hyperglycemia which has a harmful effect on all systems including the reproductive system of animals. Purpose of this study is to evaluate the effects of methanolic and aqueous extract derived from celery (Apium graveolens L.) on fertility and some hematological and biochemical parameters in alloxan-induced diabetic male rats. This study was conducted on eighty experimental male rats (Sprague Dawly strain) weighing about 220 g each were used throughout the study and randomly assigned to eight experimental groups of 10 rats each. Group I received normal saline (0.5 ml/kg) and serves as a control. Group II and III - gavaged daily for thirty days with 1ml of the ethanol extract at doses of 213 mg/kg and 425 mg/kg body wt and served as control. Group IV group Rats were made diabetic by injecting alloxan monohydrate “B.O.H chemical LTD England” intraperitoneally at a dose of 150ml/kg (dissolved in fresh normal saline) to 18h fasted rat. Every week after injection, blood was collected from the hearts of all surviving rats and blood glucose levels were determined. Rats with blood sugar levels of 200 to 450mg/100 ml were considered as diabetic and were used in the study and considered as diabetic rats. Rats of experimental groups V, IV, IIV, and IIIV were treated intraperitoneally with 1 ml of Apium graveolens ethanol extract of celery (Apium graveolens L.) at doses of (213 and 425 mg/kg, 200mg/kg of aqueous extract and 14.2 mg/kg of metformin body wt respectively once a day for thirty consecutive days, and the rats control groups were treated on the same manner with the vehicle. After thirty days of treatments, all rats were sacrificed and level of blood glucose, insulin, testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH), the weight of testis, sperm count and motility were measured. Lipid profile and some hematological parameters also measured in all experimental groups. Our results show that the treatment with ethanol, aqueous extract, and metformin to diabetic rats lead to significant increased in all measured parameters of in experimental rats except the level of LH as compared to diabetic rats, control and metformin-treated groups. Effects produced by this extract were closely similar to a standard antidiabetic drug, metformin (p<0.05) hypoglycemic effects in alloxan-induced diabetic rats, fertility, protection against body weight loss of diabetic animals and might alleviate the diabetes-induced disturbances of some biochemical, hematological and lipid profile parameters Keywords: Hyperglycemia, Alloxan, metformin, Apium graveolens L

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https://doi.org/10.31032/IJBPAS/2019/8.7.4750