INTRODUCTION: The prevalence rate of infertility is about 15% in which the couples are
unable to conceive after unprotected intercourse and male factors are considered as one of the
contributing factors in the progression of this disease. Oxidative stress is one of the major
mediators for male infertility, which cause dysfunction in sperms. It is basically the raised level
of oxygen and its related free radicals such as reactive oxygen species (ROS) that triggered the
damaging of cellular membrane in intracellular environment. Throughout the process of
oxidative stress, the increased production of reactive oxygen species overcomes the level of
antioxidants in the body system. Whereas minimum amounts of reactive oxygen species (ROS)
are necessary for the physiological process of sperm’s functions.
MATERIAL AND METHOD: Patients clinically analyzed with male infertility were included
in present study to evaluate the role of oxidative, inflammatory, HSPs, antioxidants and vitamin
D in infertile patients.. Fifty infertile patients were taken in the age group of 20-50 years were
eligible for inclusion in the study. Informed consent was obtained before been included in this study. Fiftyclinically apparently healthy individuals were included as controls.In present study
various types of inflammatory (MMP-9, IL-6) and oxidative biomarkers (8-OHdG, 4-HNE,
SOD, GSH and CAT) were determine through commercially available ELIZA kits but the levels
of MDA were estimated through spectrophotometery method.
RESULTS: Present study showed reveal a significant elevation of MDA (3.63±0.65), 8-OHdG
(1.26±0.011), Isoprostanes (91.32±12.56) and 4-HNE (20.19±4.56)in subjects as compare to
control group (0.95±0.056), (0.03±0.0023), (0.86±0.032) and (3.35±1.25) respectively.
According to the results of present study levels of inflammatory biomarkers (IL-6 and TNF-?)
also significantly increasedlike IL-6 (17.26±6.35), TNF-? (31.26±4.28) and MMP-9
(199.65±6.35) in subjects as compare to control group (6.35±1.88), (19.35±4.26) and
(41.26±4.25) respectively. The levels of antioxidantsSOD (0.06±0.008), GSH (6.58±1.55) and
CAT (4.26±1.09) were significantly low in subjects as compare to normal healthy individuals
(0.14±0.034), (9.65±2.19), (6.35±1.88) respectively. Additionally, levels of vitamin D
(13.28±2.09) disturbed in subjects as compare to control (36.55±4.19).
CONCLUSION:Conclusively, it is evident that vitamin-D exhibits positive correlation with the
male fertility by maintaining the levels of sex hormones (LH, FSH, and testosterone), by upregulating
the antioxidant defense (SOD, CAT, and GSH), and down-regulating the oxidative
stress (MDA and NO species) induced by ROS and RNS. Therefore, the levels of vitamin D are
of prime important in maintaining the sexual health of human.
Keywords: male infertility, oxidative stress, antioxidants, HSPs, spermatogenesis, apoptosis
https://ijbpas.com/pdf/2019/May/MS_IJBPAS_2019_4723.pdf
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https://doi.org/10.31032/IJBPAS/2019/8.5.4723
