INTRODUCTION: Cancer the most deadly disease nowadays and out of all types of cancers,
prostate cancer (PCa) is the second most commonly occurring cancer after skin cancer
worldwide and it is the sixth leading cause of death in men. It is mostly developed in the age
over fifty and the rate of PCa in Pakistan is 5.3 per 1 lac persons per year. Prostate cancer is the
cancer of prostate gland, whichresides under the urinary bladder, and in anterior of the rectum
and its size changes with the growing age. It develops quickly during the puberty and pumped by
the increase in androgens. These hormones include testosterone and dihydrotestosterone
(DHT).There are several risk factors involved in prostate cancer but this does not necessarily
means that a person would develop cancer if he were having one of the risk factors.
MATERIALS AND METHODS: Total hundred (100) samples were collected for present study
design and equally distributed into two groups like group A (control) consist of 50 samples of
normal healthy individuals but group B (subject) consist of fifty (50) samples of prostate cancer
suffering individuals. All of these samples were taken to evaluate the role of oxidative, inflammatory biomarkers as well as heat shock protein (HSPs) in prostate suffering individuals.
All of above-mentionedbiomarkers were performed through respective protocols. Like
isoprostane, 8-OHdG, IL-6, TNF-?, MMP-9, TGF-?, HSP-90, 70, and 27 were performed
through commercially available ELIZA kits as well as MDA were evaluate through
spectrophotometry method.
RESULTS: As shown in results the levels of MDA (nmol/ml) were high in subjects (5.19±0.99)
as compare to normal healthy individuals (0.97±0.085). Levels of oxidative biomarker like
Isoprostane (pg/ml) (84.29±4.19), 8-OHdG (pg/ml) (1.02±0.035), 4-HNE (?mol/ml)
(12.39±2.19)were also higher in subjects Vs control group (32.19±4.19), (0.18±0.019) and
(1.22±0.19) respectively. Levels of HSPs were also disturbed in positive control group like HSP-
70 (36.29±5.19), HSP-90 (21.59±2.88), and HSP-27 (17.89±3.08) as compare to negative control
group (6.99±1.99), (8.56±1.08) and (5.19±1.17) respective. Same above mention variables, the
levels of inflammatory biomarkers were also elevated in subjects.
CONCLUSION: The present study depicts that antioxidant status is decrease in prostate cancer
patients. The resulting oxidant-antioxidant imbalance proposes that patients with prostate
disorders are vulnerable to a lot of oxidative stress, which may be one of the factors responsible
for the development and progression of prostate cancer. Assessing the antioxidant levels in the
patients with these prostatic disorders may help in proper management and decreasing disease
morbidity.
Keywords: Prostate cancer, oxidative biomarkers, inflammatory biomarkers, Heat shock
proteins, matrix metalloproteases
https://ijbpas.com/pdf/2019/May/MS_IJBPAS_2019_4722.pdf
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https://doi.org/10.31032/IJBPAS/2019/8.5.4722
