ABSTRACT

The main objective of this study deals with the preparation of petroleum ether and aqueousmethanol extracts from flowers, leaves, stems and roots of Nicotiana glauca plant as well as chloroform, ethyl acetate and n-butanol fractions from Albaha region, Kingdom of Saudi Arabia. Furthermore, the obtained extracts and fractions were assessed for their antimicrobial activity against four bacterial (two Gram-positive and two Gram-negative) and two fungal reference strains, using disc diffusion method. The results of the study show that petroleum ether extract exhibited the highest activity against all the tested bacterial strains as compared to aqueousmethanol extract and among all fractions, ethyl acetate fraction possess a good sensitivity through the different strains. Tested for their antifungal properties, all tested extracts and fractions are inactive against Aspergillus flavus but they exhibited in some cases a moderate activity against Candida albicans as compared to Amphotericin B, used as positive control. Thereby, our results justify the possibility to use the obtained extracts and fractions in the treatment of some bacteria and fungi infections. Keywords: Nicotiana glauca, extracts, fractions, antimicrobial properties

https://ijbpas.com/pdf/2019/May/MS_IJBPAS_2019_4700.pdf

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https://doi.org/10.31032/IJBPAS/2019/8.5.4700