ABSTRACT

An efficient micropropagation system for strawberry (Fragaria ananassa L.) has been developed to improve strawberry production. An experiment to evaluate the effect of growth regulators under tissue culture conditions and the growth of cultured plants under hydroponic conditions was carried out in the plant tissue culture lab and hydroponic greenhouse of Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore Pakistan. For shoot induction, the sterilized strawberry seeds were cultured on Murashige and Skoog media which have different concentrations of 6-benylaminopurine viz., 0?M, 1?M, 5?M and 10?M and a constant 10?M 2,4-D (2,4-Dichlorophenoxyacetic acid). The highest average number of shoots were observed at same concentrations of 10?M BAP and 10?M 2,4-D during 4-5 days of treatment while the shoot induction was also occurred at 0?M BAP and 10?M 2,4-D concentrations. The other two concentrations 1?M and 5?M showed no effect which also caused no shoot induction. The plant grown from tissue culturing of seeds give better results under hydroponic conditions. It was concluded from our study that the growth of strawberry under controlled conditions (on MS media) and then under hydroponics may be helpful to improve plant growth and production. Keywords: micropropagation, Fragaria ananassa, hydroponic, 2,4-Dichlorophenoxyacetic acid, 6-Benzylaminopurine, growth regulators

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https://doi.org/10.31032/IJBPAS/2019/8.4.4698