ABSTRACT

Plant tissue culture is a technique, used to grow plant cell, tissue and organ under controlled and aseptic conditions, where the concentration of hormones is well defined. It is an important tool in both applied and commercial applications. Lilium candidum is an important ornamental plant also used for pharmaceutical purposes. In our present study lilies species candidum was used to grow under controlled conditions by using its leaf, shoot and root as explant to regenerate callus. The effects of change in the concentrations of growth hormones was accessed for callus regeneration that how the concentration of hormone caused a change in the growth of shoot and root from undifferentiated mass of cells or callus. It was concluded that direct organogensis occuried when we treated explant with 2-4-D (2,4-Dichlorophenoxyacetic acid) and BAP (6- Benzylaminopurine) growth regulators or hormones. Keywords: Plant tissue culture, Lilium candidum, regeneration, explants, callus, growth hormones

https://ijbpas.com/pdf/2019/April/MS_IJBPAS_2019_4697.pdf

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https://doi.org/10.31032/IJBPAS/2019/8.4.4697