ABSTRACT

Background: The pathogens like viruses and some bacteria which are reside in our blood pose a great threat and have potential to cause serious diseases. These organisms have high potential of cause’s diseases to those people who are in easy to these microbes. Such microorganisms are therefore classified as a potential occupational health hazard for Health care workers (HCWs) across the world. These pathogens are very much active in causing disease to people who are attached to the hospitals in anyway special to those people who are working inside hospitals like doctors, nurses and all other paramedical staff the Health care workers (HCWs) has mentioned a list of 20-25 organisms. These microbes are so lethal and harmful that they can even cause a disease in spite of all the most progressive equipment’s, apparatuses and other protective measures. There are some protective measures which have been suggested by HCWs to protect working staff from these easily attacking microbes especially viruses of hepatitis B and C. there are many harsh and severe results of Hepatitis C such as liver damage because of invasion of cancerous cell of live. Objectives: The current research study was designed to investigate the Hepatitis C prevalence in different hospitals of Islamabad like Military Hospital (MH), combined military hospital (CMH) and some other civil hospital such as, Quid-e-Azam Hospital and Pakistan institute of Medical Sciences (PIMS). The data was collected from different staff of the hospitals such as nurses, doctors and other staff in order to minimize the risk association of the disease Transmission. Design of Research Work: Different samples (n=400) were collected including 260 male and 140 female individuals from Islamabad in all the mentioned hospitals in the duration of nearly 8- 10 months from March 2018 to November 2018 in which Doctors, Nurses, and all other helping staff (Laboratory technicians, Operation Theatre Staff) Approximately 400 samples were collected from all the mentioned four hospitals. Results: Out of these 400 samples were selected for the study including 260 male and 140 female candidates. The general information of these subject such residence, literacy and gender are mentioned in the result section. Out of these 400 samples 290 were identified by RAID method while 110 were analyzed ELISA method. Out of these 110 samples which are examined by ELISA indicated that 14 samples showed no reactivity (Nonreactive), 94 were reactive and 02 samples were neutral (Borderline) while in case of RAPID 257 sample were nonreactive, 24 reactive and 09 samples were on borderline (Neutral). The explained illustration is present in Result section of main text. Conclusion: The research was designed to investigate the core cause of Hepatitis in Medical staff of different Hospitals of Islamabad. These results shows that the role of doctors, nurses and other medical staffs rule is crucial in preventing and controlling Hepatitis C (HCV) and some of the preventive measure must be undertaken. Keywords: ELISA, Rapid, Microbes, Viruses, Pathogen, Biosafety

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https://doi.org/10.31032/IJBPAS/2019/8.4.4710