ABSTRACT

A Study to Isolate, identify and characterize the Salmonella species along with other microbes from poultry/chicken products in Tikrit local markets and supermarkets in Iraq was carried out. A broad-range PCR assay for the detection of bacteria belonging to the Salmonella spp was developed in this study. Salmonella sps have been widely isolated from raw meats, poultry and poultry products, milk and milk products and the environment. S. Enteritidis is more prevalent in poultry and poultry products as it’s associated with “salmonellosis” in poultry. Worldwide, epidemiological reports indicate that poultry meat is the food most incriminated in food poisoning. 16s r DNA genes encoded a ribosomal RNA. Each species unique sequence of this common gene can be used as a means for identifying bacterial sps like Salmonella. Several species of Gram negative pathogenic bacteria were isolated from the poultry meat collected from various places of food markets located in Iraq used in this study. Other bacteria identified in this current study were: E.Coli, one type of Pseudomonas Spp, 6 types of Salmonella sps etc., 8 types of bacteria isolated and characterized with PCR technique; the target genes (spvB, 16s rDNA and hilA ) produced amplicons at 717bp, 572bp and 854bp which were shown to be 100% specific targeted Salmonella Sps bacteria in the collected samples. DNA was isolated from each organism and was amplified by PCR followed by purification and DNA sequencing. The resulting sequences were then analysed to allow for identification of the bacteria isolated. The molecular techniques used in this experiment were performed with the intension of illustrating the usefulness of 16s rDNA sequencing for academic institutions laboratory work in microbiology. Keywords: Poultry meat, Salmonella, PCR, 16rsDNA, bacteria, Isolation and characterization

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https://doi.org/10.31032/IJBPAS/2019/8.4.4682