ABSTRACT

BACKGROUNG Radicular cyst formation occurs from epithelial residues responsible for tooth development. Septic and necrotic dental pulp releases endotoxin which stimulates thedevelopment of radicular cyst by increasing chemokines and cytokines expression. LPS don’t cause tissue damage directly but it stimulate several cells like macrophages, neutrophils, T and B lymphocytes, plasmocytes and fibroblasts that in turn release cytokines named interleukins, interferon, TNF-?, growth factors and prostaglandins which further stimulate bone resorption. During inflammation NO is produced by LPS stimulation and inflammatory cytokines. Xanthine oxidase produces reactive oxygen species during the process of inflammation in the final product of purine metabolism and Nitrotyrosin act as an index of ONOO- in radicular cyst. Binding of RANKL to its receptor RANK stimulates proliferation, differentiation and formation of osteoclast. MATERIAL AND METHOD Forty seven (n=47) samples with radicular cyst and twenty (n=20) samples of normal pulp as control from extracted teeth were substituted in the current study. Oxidative stress biomarkers (SOD, CAT, AOPPs and AGEs), Nitric oxide (NO), Lipopolysaccharides (LPS), were assessed spectrophotometrically. While the activity of inducible nitric oxide synthase (iNOS) and Matrix metalloproteinases (MMPs)were estimated with help of their respective kit methods from tissue homogenates. RESULTS The level (nmol/ml) of MDA was significantly (p=0.033) raised in subject as compared to healthy controls (4.11±0.24 Vs 1.37±0.03).Diseased groups had higher levels of iNOS (28.16±5.09 Vs 7.98±1.88IU/ml), Xanthine Oxide (67.8±5.99 Vs 43.98±7.98 IU/mL), Nitro tyrosine (33.09±8.56 Vs 1.78±0.012nmol/L), Lipopolysaccharides (125.25±11.26 Vs 17.25±1.26pg/ml) than control one. CONCLUSION Our results indicate that xanthine oxidase and Nitrotyrosin were highly expressed in several cellular types present in radicular cyst. The expression of LPS was positively correlated with disease severity therefore it play crucial role in the formation of radicular cyst. Increased level of sRANKL and decline in OPG were responsible for bone resorption. Keywords: Matrix metalloproteinases (MMPs),Xanthine oxidase (XO), Lipopolysaccharides (LPS), Inducible nitric oxide synthase (iNOS)

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https://doi.org/10.31032/IJBPAS/2019/8.2.4656