Latest News

CORRELATION OF PRIDICTIVE VARIABLES AND THEIR INTERPLAY TO DEVELOP OXIDATIVE STRESS IN HCV PATIENTS RECEVING INTERFERON THERAPY
Authors: ARIF MALIK1* , MEHWISH IFTIKHAR3, SAIMA RUBAB KHAN2, MUHAMMAD WAQAS4, RABIA RASOOL1, RABAIL ALAM1,

ABSTRACT
BACKGROUND: Hepatitis C has become a worldwide concern because of its association with hepatocellular carcinoma and liver cirrhosis. Interferon have antiviral properties that causes modulations in the cytokines cascade that increase the synthesis of reactive oxygen species and result in oxidative stress and is associated with many inflammatory conditions, which patients develop soon after the initiation of therapy. AIMS: To assess the oxidative stress in hepatitis C patients after receiving interferon therapy. METHODOLOGY: For the current study, blood samples of the Hepatitis C patients who received interferon therapy (n=100) and healthy individuals were selected (n=100). Plasma was tested for stress markers by their respective spectrophotometric protocols. ELISA kits were used for AOPPs and AGEs. RESULTS: The results of present study demonstrate significantly higher levels of MDA and AOPPs and AGEs along with reduced levels of GSH that support the presence of a pro-oxidant state in Hepatitis C patients after therapy. Level of MDA, AOPPs and AGEs in subjects were significantly increased (3.88±1.06 nmol/ml, 1.32±0.31 ? mol/g and 3.77±0.27 ? mol/g) as compared to the healthy control group (1.43±0.36 nmol/ml, 0.849±0.04 ? mol/g and 2.56±0.10 ? mol/g). Whereas, levels of GSH, CAT, GPX and GRX decreased significantly (3.12±1.40 ?g/ml, 1.51±1.15 nmol/mol Prot., 6.53±0.46 U/L, and 3.40 ±0.41U/L) as compared to healthy controls (9.79±1.22 ?g/ml, 3.95±0.81 nmol/mol Prot., 8.22±0.36 U/L, 1.47±0.22U/L) CONCLUSION: Hepatitis C patients who received interferon therapy exhibit low levels of antioxidants along with increased protein and lipid peroxidation due to presence of inflammation. Interferon therapy in conjunction with antioxidants and micro nutrients like zinc, iron etc. can help achieve better outcomes by counteracting the free radicals. Keywords: Hepatitis C, MDA, IL-1, TNF?, IFN?, AOPPs, AGEs

    https://ijbpas.com/pdf/2019/February/MS_IJBPAS_2019_4655.pdf
Download PDF
https://doi.org/10.31032/IJBPAS/2019/8.2.4655

Gallery