ABSTRACT

INTRODUCTION: Osteoporosis, also known as silent thief, is a noteworthy condition in which mineral ions that are important for bone structure loose gradually. Deficiency of active vitamin D and down-regulation of osteoprotegrin leads to the bone mineralization through maintaining the activity of osteoclastic and osteoblastic cells. AIM: To evaluate the differential expression of prophetic variables of medical importance and their interplay in the development of osteoporosis. MATERIAL AND METHOD: Thirty healthy females and fifty osteoporotic females screened at Ganga Ram hospital Lahore were included in the present study. Serum was evaluated for the levels of 8-hydroxy-2-deoxyguanosine, isoprostanes, vitamin D, Malondialdehyde, nitric oxide, superoxide dismutase, catalase and glutathione via their respective spectrophotometric and ELISA methods. RESULTS: The present study has reported that the levels of stress markers were significantly higher in the osteoporotic females. Levels of MDA, 8-OHdG, isoprostanes and NO were significantly higher in the females with osteoporosis (2.59±0.15 nmoles/ml, 0.326±0.01 pg/ml, 22.06±2.26 pg/ml and 57.91±3.93 ?mol/L) as compared to healthy females (0.95±0.13 nmoles/ml, 0.02±0.001 pg/ml, 0.99±0.13 pg/ml and 19.46±1.38 ?mol/L), while the levels of antioxidants such as SOD, CAT, GSH and vitamin D were significantly lower in osteoporotic femalesin comparison to control group. CONCLUSION: the current study suggests a significant role of calcium under the influence of vitamin D as well as several oxidative markers and inflammatory cytokines i.e., IL-7, sRANKL and OPG in the progression of osteoporosis. Up-regulation of sRANKL and down-regulation of OPG are involved in the bone resorption by the activation of osteoclast cells. Keywords: Oxidative stress biomarkers, Osteoporosis, serum Receptor Activator of Nuclear factor

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https://doi.org/10.31032/IJBPAS/2019/8.2.4654