ABSTRACT

Sulfotransferases (SULTs) family plays a significant role in the biotransformation of xenobiotics and endogenous carcinogenic and mutagenic compounds. Recent data identified various genetically polymorphic SULTs enzymes with significant variations in the enzyme activity. This study aimed to investigate the impact of SULT1A1 gene polymorphism and its potential risk on females with breast cancer in Jordan using a PCR-RFLP and Sanger Sequencing methods. The analysis showed that 24.7% of the patients and 25.3% of the controls were homozygous for the SULT1A1*1 allele (SULT1A1*1/SULT1A1*1) compared to 8.8% and 5.7% homozygous for the SULT1A1*2 allele (SULT1A1*2/SULT1A1*2) for patients and controls respectively. Most of the patients and controls were heterozygous for SULT1A1*1 allele (SULT1A1*1/SULT1A1*2) with rates of 66.5% and 69.0% 27 in patients and controls respectively. In addition, the frequencies of the mutant SULT1A1*2 allele were 0.42 and 0.4 in the patient and control groups respectively. No significant difference in genotype and allele distribution was noted between the breast cancer and control groups. The risk of breast cancer in individuals carrying the SULT1A1*2 allele was determined by combining the SULT1A1*1/SULT1A1*2 and SULT1A1*2/SULT1A1*2 genotypes. No association was observed 32 between SULT1A1 polymorphism and breast cancer incidence (P = 0.63; OR, 0.93; 95% CI, 0.68–1.26). 33 However, SULT1A1*2 allele was found to increase the risk of breast cancer by 1.26-fold. . Keywords: Polymorphism; breast cancer; SULT1A allele, DNA sequencing

https://ijbpas.com/pdf/2019/February/MS_IJBPAS_2019_4633.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2019/8.2.4633