ABSTRACT

Silver nanoparticles (Ag NPs) with particle size 42-44nm function as a fast and sensitive marker for detection of histamine resulting from biological samples of TB and HIV patients. Characterization of AgNPs is carried out by using different analytical techniques such as UV–vis, FTIR, and TEM. The possibility of AgNPs, a nanomaterial pervasively used in nanotechnology and pharmaceutical industries, to directly induce histamine secretion without prior allergen sensitization has remained uncertain. Our investigation suggests that systemic circulation of Ag NPs may prompt histamine release at different locales causing abnormal changes in the secretion of histamine, which regulates immunity in TB and HIV infected patents. The Mycobacterium tuberculosis (Mtb) has developed methods to evade the immune system and survive. With the discovery of nanoparticle (NP)-based drugs, it is necessary to research their antimycobacterial properties and bactericidal mechanisms. In this study, we synthesized mixed AgNPs and tested their ability to inhibit Mtb growth into macrophages and investigated the cytotoxic effects of NPs in THP-1 cells. The in vitro experiment, involving 20 strains of the tuberculosis mycobacteria, exhibited a potentiating effect of silver nanoparticles on known antituberculous preparations in respect of overcoming drugresistance of the causative agent. Keywords: Histamine, TB, HIV, Nanoparticles

https://ijbpas.com/pdf/2019/February/MS_IJBPAS_2019_4630.pdf

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https://doi.org/10.31032/IJBPAS/2019/8.2.4630