ABSTRACT

This study assessed the antimicrobial activity of biosynthesized AgNPs with an average size of 64 nm on two hospital strains of S. aureus and Pseudomonas aeruginosa resistant to a large number of antibiotics and a reference strain from a culture collection and a perspective for application of nanosilver with antibiotics to enhance antimicrobial activity. The prevalence of selected pathogens S. aureus and P. aeruginosa in 24 samples of wound swabs and ear infection samples submitted to the Microbiological Laboratory of Govt General Hospital, Guntur during the period Jan 2018-Oct 2018 was investigated. Results showed the potential of using prepared AgNPs along with selected antibiotics as an alternative to conventional antimicrobial agents that are currently used. Root-extract from Achyranthes aspera natural plant was used as a precursor for the synthesis of silver-nanoparticle material, which was characterised by a scanning electron microscopy having Energy Dispersion Spectroscopy (SEM EDS), XRD for particle size determination, UV-Visible spectroscopy for stability studies, FT IR analysis for sunctional group determination The main objective of this contribution was to prepare AgNPs from root extract and evaluate their antimicrobial activity on S. aureus and P. aeruginosa bacterial isolated from clinical samples considered as drug-resistant bacteria by minimum inhibitory concentration (MIC) method. Keywords: Bio Synthesized Silver Nanoparticles, S. aureus and P. aeruginosa

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https://doi.org/10.31032/IJBPAS/2019/8.2.4628