ABSTRACT

The current research work was undertaken to identify, isolate and characterization of microbial quality of food, dining table surface and kitchen surface of ten popular restaurants in various location of Baghdad city in Iraq during January to December 2016 period. Total 50 samples, seven categories of food and surface samples were collected and cultured in selected medium to isolate the bacterial count and identified as per ht morphological and biochemical characteristics. The result revealed that 14 types of bacterial species including seven gram positive and seven gram negative bacterial isolates namely Acinetobacter coloaceticus, Bacillus cereus, Bacillus mycoides, Bacillus mycoides, Campylobacter Spp, Clostridium perfringens, E. coli , Enterococcus fecalis, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella Spp, Staphyl coccus capre and Staphylococcus aureus respectively identified from food samples (Cake-9 sample, Chicken Curry-3 sample, Dal-7 samples, Rice-6 samples and Ruti -1 sample) and surface samples (Dining Table Top/Kitchen surfaces-24 samples). The antibiotics susceptibility pattern results showed all of these isolates were resist to most traditional antibiotics but in different ratios. DNA was isolated from each organism isolated from the current study and was amplified by PCR followed by purification and DNA sequencing. The resulting sequences then analyzed to allow for identification of the bacteria isolate. The study concluded that preventive and disinfectant plans should be considered to ensure contamination free restaurants for the better health of all consumers. Keywords: Characterization of pathogenic bacteria, food quality, Iraq

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https://doi.org/10.31032/IJBPAS/2019/8.1.4621