ANTIBACTERIAL ACTIVITY OF WATER HYACINTH (Eichhornia crassipes) EXTRACTS AGAINST Aeromonas hydrophila IN-VITRO AND IN-VIVO
Authors: Janet O. Saturno , MISSAEL T. MAXION AND RODORA M. BARTOLOME

ABSTRACT
This paper demonstrated the antibacterial activity water hyacinth (Eichhornia crassipes) extracts against Aeromonas hydrophila in-vitro and in-vivo. The active components of the plant were obtained using ethanolic, methanolic, and water as extracting solvents. Among extracts, ethanol as extracting solvent recorded the highest zone of inhibition at 11.33mm, which is not significantly different to water extract (9.33mm). In addition, determination of the activity index from the in-vitro antibacterial experiments revealed that ethanolic (0.723) and water (0.596) extracts exhibited significant activity. The test bacteria are moderately susceptible to both extracts. In contrast, methanol extract had the lowest antibacterial activity. However, in-vivo assay revealed no significant difference in terms of survival between the treated feeds with positive control (90.333) and the feeds treated with ethanol extract (86.667). The present study found that water hyacinth exhibits potential as an antibiotic agent in aquaculture. Keywords: Aeromonas hydrophila, Solvent extraction, Susceptibility testing, Water Hyacinth (Eichhornia crassipes)

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https://doi.org/10.31032/IJBPAS/2019/8.1.4609