ANTIBACTERIAL ACTIVITY OF WATER HYACINTH (Eichhornia crassipes) EXTRACTS AGAINST Aeromonas hydrophila IN-VITRO AND IN-VIVO Authors: Janet O. Saturno , MISSAEL T. MAXION AND RODORA M. BARTOLOME
ABSTRACT
This paper demonstrated the antibacterial activity water hyacinth (Eichhornia
crassipes) extracts against Aeromonas hydrophila in-vitro and in-vivo. The active
components of the plant were obtained using ethanolic, methanolic, and water as extracting
solvents. Among extracts, ethanol as extracting solvent recorded the highest zone of
inhibition at 11.33mm, which is not significantly different to water extract (9.33mm). In
addition, determination of the activity index from the in-vitro antibacterial experiments
revealed that ethanolic (0.723) and water (0.596) extracts exhibited significant activity. The
test bacteria are moderately susceptible to both extracts. In contrast, methanol extract had the
lowest antibacterial activity. However, in-vivo assay revealed no significant difference in
terms of survival between the treated feeds with positive control (90.333) and the feeds
treated with ethanol extract (86.667). The present study found that water hyacinth exhibits
potential as an antibiotic agent in aquaculture.
Keywords: Aeromonas hydrophila, Solvent extraction, Susceptibility testing, Water
Hyacinth (Eichhornia crassipes)